HPLC METHOD DEVELOPMENT

💻𝗖𝗢𝗨𝗥𝗦𝗘 𝗦𝗨𝗠𝗠𝗔𝗥𝗬: ✅The "HPLC Method Development Blueprint" course is a comprehensive training program designed to master HPLC technique. This course offers an in-depth exploration of the working principles of various LC techniques, guiding participants through the critical process of HPLC method development. ✅It covers the selection criteria for HPLC columns based on interaction, dimension, pore size, and ligand, especially for small molecules, proteins and peptides, mobile phase selection- including solvents, buffers, and pH levels. ✅The role of pH and pKa in method development is thoroughly examined, including methods for determining pKa through UV spectroscopy and potentiometry. ✅The course addresses common challenges in developing methods for related substances, gradient elution, and strategies to overcome problems like peak splitting, column degradation, and carryover effects. Special attention is given to the principles of chiral separation and the use of protein-based columns for this purpose. ✅The course also includes practical tips for achieving a smooth baseline during gradient runs and retaining acidic and basic compounds effectively. ✅The course features live session recordings from various dates, providing real-life examples and case studies. These sessions, including those focusing on QbD & AQbD, offer participants a unique opportunity to see HPLC method development in action, discuss common issues, and learn from experienced practitioners. 𝗖𝗘𝗥𝗧𝗜𝗙𝗜𝗖𝗔𝗧𝗘: 🎓Receive a certificate upon successfully finishing this course 𝗖𝗢𝗨𝗥𝗦𝗘 𝗗𝗨𝗥𝗔𝗧𝗜𝗢𝗡: ⏳1268 Minutes (21.13 Hours) 𝗟𝗔𝗡𝗚𝗨𝗔𝗚𝗘: 📖English 𝗦𝗘𝗟𝗙 𝗣𝗔𝗖𝗘𝗗 𝗟𝗘𝗔𝗥𝗡𝗜𝗡𝗚: 🌏 Offered as an online course featuring pre-recorded videos and structured for distance learning, this program is available to participants globally. 𝗟𝗜𝗙𝗘𝗧𝗜𝗠𝗘 𝗔𝗖𝗖𝗘𝗦𝗦: 🎧You will have lifetime access to the course