top of page

Why does GC inlet liner have a hole?

Hello and welcome to this informative discussion. I'm Bhaskar Napte from Pharma Growth Hub, and today we'll delve into the intriguing world of Gas Chromatography liners and their unique features. In particular, we'll unravel the mystery of why some GC liners have holes.

In the realm of Gas Chromatography, there are various types of liners, each with its own distinct characteristics. You might have come across straight liners, those with tapers, double taper liners like the one shown here, liners with holes, and even cyclo liners. Today, our focus is on liners with holes, and the purpose behind these intriguing features.

Before we dive into the specifics, let's first explore the different types of injections in Gas Chromatography.

1. Split Injection:

The first one is the "split" injection, a term you're likely familiar with. Split injections are typically used when there's a high concentration of analytes in the sample and no need for low detection. As illustrated in the diagram, a portion of the sample, along with the carrier gas, enters the column based on the selected split ratio, and the rest is vented out. This results in a high flow rate, which is characteristic of split injections.

2. Splitless Injection:

The second injection type is the "splitless" injection, usually preferred when dealing with trace-level analyses and low sample concentrations. Utilizing a deactivated liner in splitless injections enhances analytical performance. In this mode, the sample comes into contact with the liner's bottom seal, and after the injection is complete, any remaining solvent vapors are vented out through the split vent.

3. Direct Injection:

The third injection technique is the "direct injection." It's employed for trace-level determinations when sample concentrations are extremely low. However, it should be used with caution, especially if there's a possibility of sample component degradation or absorption onto the injector port body, which can lead to inaccurate analyses. In direct injection, the sample is injected directly into a hot inlet, where it vaporizes due to the high temperature. These vaporized samples are then transferred into the GC column.

Now, let's focus on the peculiar liners with holes. Why are they necessary in splitless and direct injections? Direct injections and splitless injections often require large sample volumes and electronic pressure control (EPC). Since we use direct and splitless injections for trace-level component analysis, we often need to inject larger volumes, typically around 4 to 5 microliters. This is where EPC comes into play.

To comprehend the significance of EPC, let's consider the nature of direct or splitless injections. In direct or splitless modes, the inlet gas flow is very low, and it takes time for the vaporized sample to move from the injector port to the column. During this transfer, the vaporized sample tends to disperse, leading to a wider analyte bandwidth. A broader bandwidth can result in peak distortion, wider peaks at the base, and compromised resolution. To address this issue, EPC increases the flow rate temporarily during the injection, compressing the sample band into a thinner cloud. This ensures sharper peaks, reduced tailing, and improved resolution, and is referred to as electronic pressure control.

However, in traditional direct or splitless injection liners, there is no downstream flow to the split vent, as the liner creates a leak-free connection with the GC column. This can lead to pressure differences between the upstream and downstream sensors, resulting in high-pressure malfunctions. To prevent this, a small hole in the liner allows a portion of the carrier gas to escape, equalizing the pressure at the second sensor and eliminating pressure malfunctions.


In conclusion, systems equipped with electronic pressure control (EPC) require a hole in the inlet liner body, especially when using direct injections and splitless injections. Liners with a hole at the top are preferable when compounds of interest elute away from the solvent peak or when the sample contains an aqueous diluent. Liners with a hole at the bottom are best suited when dealing with tailing solvent peaks, improving resolution. Additionally, the hole in the side of the liner allows the injector to be operated in traditional split or splitless mode, providing flexibility in analytical methods.

Thank you for your attention, and I'd love to hear your thoughts on this intriguing topic.

Top of Form

Recent Posts

See All


Avaliado com 0 de 5 estrelas.
Ainda sem avaliações

Adicione uma avaliação
bottom of page