The concentration of the standard and sample is one of the most critical information to perform various method validation parameters. The inaccurate analyte concentration may lead to deviation or rework. How one can define the required concentration of sample/standard for the validation parameters? Let us explore…. Join the WhatsApp group of Pharma Growth Hub to receive more updates: https://chat.whatsapp.com/K7oqA0Rlh6hGDN2kAAnW22 Share your email in the comment below to receive this conc. matrix spreadsheet Regards Bhaskar #methodvalidation #analyticalcalchemistry #training #interview #PharmaGrowthHub

Chromatography of charged analytes is often challenging as they are not retained effectively on the commonly used reversed-phase columns. Ion pair chromatography (IPC) is a suitable alternative for chromatography of polar or ionic species. Let us understand the retention of charged analytes in IPC. Jon the WhatApp group of Pharma Growth Hub: https://chat.whatsapp.com/GyOUBdaVqGRDMK1HhkWl2u Join my YouTube channel: https://www.youtube.com/channel/UCLOirac2M8MA2vw7AHglOTQ Regards Bhaskar #IPC #RPLC #HPLC #retentionmechanism #PharmaGrowthHub #methoddevelopment

As per ICH Q2(R1), the correlation coefficient, y-intercept, slope of the regression line and residual sum of squares should be evaluated for linearity. Is the response ratio necessary for linearity? Join WhatsApp group: https://chat.whatsapp.com/K7oqA0Rlh6hGDN2kAAnW22 Regards Bhaskar #validation #linearity #responseratio #PharmaGrowthHub

The inlet liner isolates the sample from the metal walls of the injector block. The vaporization, speed of the sample, and backflash are some of the routine challenges for GC. How does an inlet liner with a “hole” helps in resolving some of these challenges? Let us explore… Join the WhatsApp group of Pharma Growth Hub: https://chat.whatsapp.com/IEtx6DfWjqPC0Nia6Vz4Iv Regards Bhaskar #GC #inletliner #methoddevelopment #analyticalchemistry #pharmagrowthhub

Which Solvent is Better for Chromatography: Methanol or Acetonitrile? Let us explore… Join the WhatsApp group for more updates: https://chat.whatsapp.com/GyOUBdaVqGRDMK1HhkWl2u Subscribe to my YouTube channel: https://www.youtube.com/channel/UCLOirac2M8MA2vw7AHglOTQ Regards Bhaskar #rplc #methaol #acetonitrile #HPLC #methoddevelopment #PharmaGrowthHub

As per RPLC principles, retention of protonated base decreases at low pH Is it possible to increase the retention of protonated bases with low pH? As per a few studies, it is possible… How? Let us explore… Jon the WhatApp group of Pharma Growth Hub: https://chat.whatsapp.com/GyOUBdaVqGRDMK1HhkWl2u Join my YouTube channel: https://www.youtube.com/channel/UCLOirac2M8MA2vw7AHglOTQ Regards Bhaskar #RPLC #HPLC #retentionmechanism #PharmaGrowthHub #methoddevelopment

Quick and effective Tips for HPLC column cleaning Join the WhatsApp group for more updates: https://chat.whatsapp.com/GyOUBdaVqGRDMK1HhkWl2u Subscribe to my YouTube channel: https://www.youtube.com/channel/UCLOirac2M8MA2vw7AHglOTQ Regards Bhaskar #rplc #HPLC #methoddevelopment #PharmaGrowthHub #columncleaning

FDA and EMA have agreed upon a “Carcinogenic Potency Categorization Approach” to predict the AI Limits for Nitrosamines and NDSRIs. The Nitrosamines are categorized into 5 different Potency Categories based on α hydrogen score, deactivating features score, and activating features score. Both FDA have provided a few configurations for calculating the α hydrogen score, whereas, a few are missing. ▶️Follow this link to join my WhatsApp group: https://chat.whatsapp.com/Jrem91dAOsG3Aob4kRQpZd Regards Bhaskar #nitrosamine #NDSRI #AI #CPCA #EMA #FDA #PharmaGrowthHub #riskassessment