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Why You Must Avoid Highly Aqueous Mobile Phases When Using C8/C18 Columns?
This article delves into the challenges due to the usage of high aq. mobile phases in RPLC and offers solutions
bhaskar napte
Dec 6, 20233 min read
2,121
1
How to Select the Dimension of an HPLC Column?
Selecting the right HPLC column dimensions during HPLC method development
bhaskar napte
Dec 1, 20232 min read
1,965
3
How to reduce mobile phase consumption during an HPLC analysis
How to reduce mobile phase consumption during HPLC analysis
bhaskar napte
Nov 30, 20233 min read
753
1
💥Effect of Triethylamine (TEA) on the Retention in RPLC
Impact of Triethylamine on Retention, and Peak Tailing in HPLC analysis
bhaskar napte
Nov 27, 20232 min read
3,527
2
What is the effect of free silanols in RPLC and how to reduce it?
Understand the silanol effect and way out to minimize it in RPLC
bhaskar napte
Nov 23, 20232 min read
1,532
1
Are the Tailing factor, Symmetry factor, and Asymmetry factor the same?
tailing factor assymetry factor and symmtery factor as per USP and EP or Ph. Eur
bhaskar napte
Nov 15, 20232 min read
5,220
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Why resolution and plate number is measured by using peak width at the half height
measurement of plate number and resolution in HPLC analysis
bhaskar napte
Nov 10, 20233 min read
226
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Maximizing HPLC Column Efficiency: A Comprehensive Guide
This blog will help you to maximize the performance of your HPLC column
bhaskar napte
Nov 8, 20234 min read
471
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Peak Tailing: Phenomenon, Symptoms and Corrections
Peak tailing phenomenon, symptoms, and corrections
bhaskar napte
Nov 7, 20233 min read
2,545
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Mastering Peak Compression: The Key to Sharper Chromatography
Hoe gradient elution can help in peak compression
bhaskar napte
Nov 1, 20233 min read
222
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pH, pKa, and Retention
The role of pH and pKa on retention of acid and base in RPLC
bhaskar napte
Oct 30, 20234 min read
3,280
2
Amino Columns in Chromatography
Use of amino column in HPLC method development
bhaskar napte
Oct 20, 20231 min read
103
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Retention of Acidic, Basic, and Neutral Compounds in Ion Pair Chromatography
Retention of Acidic, Basic, and Neutral Compounds in Ion Pair Chromatography
bhaskar napte
Oct 20, 20233 min read
151
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Exploring the Surprising Increase in Retention Time of Protonated Bases at Low pH in RPLC
Exploring the Surprising Increase in Retention Time of Protonated Bases at Low pH in RPLC
bhaskar napte
Oct 20, 20232 min read
28
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Why does Acetonitrile have higher elution strength than methanol?
We will explore, why Acetonitrile has higher elution strength than methanol.
bhaskar napte
Oct 20, 20232 min read
4,751
3
Phenyl Column Mystery
The role of Phenyl column in HPLC method development
bhaskar napte
Oct 20, 20232 min read
205
1
Unraveling the Advantages of Core-Shell Columns in HPLC
what core-shell particles are, how they differ from traditional porous particles, their history, and the advantages they bring
bhaskar napte
Oct 20, 20232 min read
103
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Methanol vs. Acetonitrile: Which Solvent is Better for Chromatography?
we'll explore the unique properties of methanol and acetonitrile and discuss when and why you might prefer one over the other for HPLC
bhaskar napte
Oct 20, 20232 min read
768
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QbD vs AQbD
Understanding the key terms associated with QbD (Quality by Design) and AQbD (Analytical Quality by Design)
bhaskar napte
Oct 20, 20232 min read
2,005
2
Efficient Tips for Column Cleaning in Reverse Phase Liquid Chromatography
highly effective tips for column cleaning, particularly geared toward reverse-phase liquid chromatography
bhaskar napte
Oct 20, 20232 min read
197
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